Cryo-Electron Tomography

Cryo-Electron Tomography

Structural Biology in situ

Briegel, Ariane; Foerster, Friedrich

Springer International Publishing AG

06/2024

389

Dura

9783031511707

Pré-lançamento - envio 15 a 20 dias após a sua edição

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1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography.- 2. Principles of tomographic reconstruction .- 3. Cryo-preparation for cellular tomography.- 4. Cryo-FIB milling.- 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques.- 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM.- 7. Data collection.- 8. Subvolume averaging and classification.- 9. High resolution tomography/ Molecular model building and visualization.- 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography.- 11. Tomogram segmentation.- 12. Electron tomography of cryo-fixed and resin-embedded samples.- 13. Democratization of tomographic data / FAIR data.
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electron optics;phase contrast;tomography;tomographic reconstruction;Fourier-slice theorem;cryo-preparation techniques;cellular tomography;Cryo-FIB milling;electron and ion microscopy;Imuno labeling;DNA origami;origami nanostructures;DNA origami and light microscopy;cryo tomography data collection;Cryo-ET and mass spectrometry;high resolution tomography