Transmembrane ?-Barrel Proteins
portes grátis
Transmembrane ?-Barrel Proteins
Methods and Protocols
Ieva, Raffaele
Springer-Verlag New York Inc.
03/2024
386
Dura
9781071637333
15 a 20 dias
Descrição não disponível.
The Name is Barrel, ?-Barrel.- Recombinant Expression and Overproduction of Transmembrane b-Barrel Proteins.- Bacterial Envelope Fractionation.- Fluorescent Labeling of Outer Membrane Proteins Using the SpyCatcher-SpyTag System.- In Vitro Reconstruction of Bacterial ?-Barrel Membrane Protein Assembly Using E. coli Microsomal (Mid-Density) Membrane.- Examining Protein Translocation by ?-Barrel Membrane Proteins Using Reconstituted Proteoliposomes.- In Vivo Disulfide-Bond Crosslinking to Study ?-Barrel Membrane Protein Interactions, Dynamicity, and Folding Intermediates.- Site-Specific Photocrosslinking to Investigate Toxin Delivery Mediated by the Bacterial ?-Barrel Assembly Machine.- Analysis of Transmembrane ?-Barrel Proteins by Native and Semi-Native Polyacrylamide Gel Electrophoresis.- Affinity Purification of Membrane ?-Barrel Proteins via Biotin-Tagged Peptidiscs.- Monitoring the Interaction of the Peptidoglycan with the Bacterial ?-Barrel Assembly Machinery.- MitoLuc:A Luminescence-Based Assay to Study Real-Time Protein Import into Mitochondria.- Modular Assembly of Mitochondrial ?-Barrel Proteins.- Tracking the Activity and Position of Mitochondrial ?-Barrel Proteins.- Conformational Heterogeneity of ?-Barrel Membrane Proteins Observed In Situ Using Orthogonal Spin Labels and Pulsed ESR Spectroscopy.- Characterization of ?-Barrel Outer Membrane Proteins and Their Interactions with Chaperones by Chemical-Crosslinking Mass Spectrometry.- Dissecting the Organization of a ?-Barrel Assembly Machinery (BAM) Complex by Neutron Reflectometry.- Expression, Purification, and Cryo-EM Structural Analysis of an Outer Membrane Secretin Channel.- Recent Advances in Modeling Membrane ?-Barrel Proteins Using Molecular Dynamics Simulations: From Their Lipid Environments to Their Assemblies.- Structural Modeling of T9SS Outer Membrane Proteins and Their Complexes.- Rationale in Custom Design of Transmembrane ?-Barrel Pores.- Stress-Based Screening for Compounds that Inhibit ?-Barrel Outer Membrane Protein Assembly in Gram-Negative Bacteria.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
Transmembrane beta-barrel;Mitochondrial targeting;Bacteria;Mitochondria;Protein assembly;Folding;Structural characterization
The Name is Barrel, ?-Barrel.- Recombinant Expression and Overproduction of Transmembrane b-Barrel Proteins.- Bacterial Envelope Fractionation.- Fluorescent Labeling of Outer Membrane Proteins Using the SpyCatcher-SpyTag System.- In Vitro Reconstruction of Bacterial ?-Barrel Membrane Protein Assembly Using E. coli Microsomal (Mid-Density) Membrane.- Examining Protein Translocation by ?-Barrel Membrane Proteins Using Reconstituted Proteoliposomes.- In Vivo Disulfide-Bond Crosslinking to Study ?-Barrel Membrane Protein Interactions, Dynamicity, and Folding Intermediates.- Site-Specific Photocrosslinking to Investigate Toxin Delivery Mediated by the Bacterial ?-Barrel Assembly Machine.- Analysis of Transmembrane ?-Barrel Proteins by Native and Semi-Native Polyacrylamide Gel Electrophoresis.- Affinity Purification of Membrane ?-Barrel Proteins via Biotin-Tagged Peptidiscs.- Monitoring the Interaction of the Peptidoglycan with the Bacterial ?-Barrel Assembly Machinery.- MitoLuc:A Luminescence-Based Assay to Study Real-Time Protein Import into Mitochondria.- Modular Assembly of Mitochondrial ?-Barrel Proteins.- Tracking the Activity and Position of Mitochondrial ?-Barrel Proteins.- Conformational Heterogeneity of ?-Barrel Membrane Proteins Observed In Situ Using Orthogonal Spin Labels and Pulsed ESR Spectroscopy.- Characterization of ?-Barrel Outer Membrane Proteins and Their Interactions with Chaperones by Chemical-Crosslinking Mass Spectrometry.- Dissecting the Organization of a ?-Barrel Assembly Machinery (BAM) Complex by Neutron Reflectometry.- Expression, Purification, and Cryo-EM Structural Analysis of an Outer Membrane Secretin Channel.- Recent Advances in Modeling Membrane ?-Barrel Proteins Using Molecular Dynamics Simulations: From Their Lipid Environments to Their Assemblies.- Structural Modeling of T9SS Outer Membrane Proteins and Their Complexes.- Rationale in Custom Design of Transmembrane ?-Barrel Pores.- Stress-Based Screening for Compounds that Inhibit ?-Barrel Outer Membrane Protein Assembly in Gram-Negative Bacteria.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
