Biophysics of Membrane Proteins

Biophysics of Membrane Proteins

Methods and Protocols

Goldman, Adrian; Postis, Vincent L. G.

Springer-Verlag New York Inc.

02/2022

336

Mole

Inglês

9781071607268

15 a 20 dias

662

Descrição não disponível.
Membrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells.- Quantifying the Interaction of Phosphite with ABC Transporters: MicroScale Thermophoresis and a Novel His-tag Labeling Approach.- Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes.- Functional Characterization of SLC transporters using Solid-Supported-Membranes.- Thermostability Assays Provide a Generic and Versatile Tool for Studying the Functional and Structural Properties of Membrane Proteins in Detergents.- Direct monitoring of GPCR reconstitution and ligand binding activity by Plasmon Waveguide Resonance.- Examining Membrane Proteins by Neutron Scattering.- Solution X-ray Scattering for Membrane Protein.- Interpreting SAXS/WAXS Data with Explicit-Solvent Simulations: A Practical Guide.- Determining the Free Energies of Outer Membrane Proteins in Lipid Bilayers.- Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry.- Determination of the Molecular Mass of Membrane Proteins Using Size Exclusion Chromatography with Multi-angle Laser Light Scattering (SEC-MALLS).- Dynamics of Membrane Proteins Monitored by Single-molecule Fluorescence across Multiple Time Scale.- Short-Range Distances Measurement by Transition Metal Ion FRET.- PELDOR/DEER - An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers.






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SEC-MALLS;cryoEM;Free Electron Lasers;MD simulations;neutron scattering