Nanopore Technology

Nanopore Technology

Methods and Protocols

Fahie, Monifa A.V.

Springer-Verlag New York Inc.

09/2021

231

Mole

Inglês

9781071608081

15 a 20 dias

470

Descrição não disponível.
Preparation of Fragaceatoxin C (FraC) Nanopores.- Preparation of Cytolysin A (ClyA) Nanopores.- Building Synthetic Transmembrane Peptide Pores.- Design and Assembly of Membrane-Spanning DNA Nanopores.- Determining the Orientation of Porins in Planar Lipid Bilayers.- Revelation of Function and Inhibition of Wza through Single Channel Studies.- Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore.- Nanopore Enzymology to Study Protein Kinases and their Inhibition by Small Molecules.- A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore.- Oligonucleotide-Directed Protein Threading through a Rigid Nanopore.- Unfolding and Translocation of Proteins through an Alpha-Hemolysin Nanopore by ClpXP.- Simulation of pH-Dependent, Loop-Based Membrane Protein Gating Using Pretzel.- Free Energy Minimization for Vesicle Translocation through a Narrow Pore.- Single Ion-Channel Analysis in Droplet Interface Bilayer.- Continuous and Rapid Solution Exchangein a Lipid Bilayer Perfusion System Based on Droplet-Interface Bilayer.- Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip.
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Protein analytes;Single molecule detection;DNA sequencing;Membrane channels;Nanopore sensing;Translocation