Exocytosis and Endocytosis

Exocytosis and Endocytosis

Methods and Protocols

Gasman, Stephane; Vitale, Nicolas; Niedergang, Florence

Springer-Verlag New York Inc.

11/2021

329

Mole

Inglês

9781071610466

15 a 20 dias

655

Descrição não disponível.
Stoichiometry of Receptors at the Plasma Membrane during Their Endocytosis using Total Internal Reflection Fluorescent (TIRF) Microscopy Live Imaging and Single Molecule Tracking.- Measuring Endocytosis During Proliferative Cell Quiescence.- Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocytic Vesicle Distribution In Adrenal Chromaffin Cells.- Quantitative Methods to Study Endocytosis and Retrograde Transport of Cargo Proteins.- High-content Drug Discovery Screening of Endocytosis Pathways.- Methods for Monitoring Endocytosis in Astrocytes.- Monitoring Activity-Dependent Bulk Endocytosis in Primary Neuronal Culture Using Large Fluorescent Dextrans.- Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretion.- High-throughput Screening for Insulin Secretion Modulators.- Different Approaches to Record Human Sperm Exocytosis.- Bovine Chromaffin Cells: Culture and Fluorescent Assay for Secretion.- Measurement of Exocytosis in Genetically Manipulated Mast Cells.- Super-Resolution Microscopy and Particle Tracking Approaches for the Study of Vesicular Trafficking in Primary Neutrophils.- An Approach to Monitor Exocytosis in White Adipocytes.- Amperometry in Single Cells and Tissue.- Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging In Mouse Adrenal Chromaffin Cells.- RUSH-synchronized Secretion to Measure Local Exocytosis.- Combining Single Molecule Super-Resolution Imaging Techniques to Unravel the Nanoscale Organization of the Presynapse.- Induction of Ca2+ Dependent Exocytotic Processes by Laser Ablation of Endothelial Cells.- Transmission Electron Microscopy and Tomography on Plasma Membrane Sheets to Study Secretory Docking.- Spatial and Temporal Aspects 0f Exocytosis Studied on the Isolated Plasma Membranes.
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Amperometry;SNARE;Proteomics;Clathrin;flow cytometry;macropinocytosis