Brown Adipose Tissue

Brown Adipose Tissue

Methods and Protocols

Guertin, David A.; Wolfrum, Christian

Springer-Verlag New York Inc.

02/2022

309

Mole

Inglês

9781071620892

15 a 20 dias

Descrição não disponível.
Brown Adipose Tissue: A Short Historical Perspective.- Brown Fat Anatomy in Humans and Rodents.- Analysis of Thermogenesis Experiments with CalR.- A Clearing Method for Three-Dimensional Imaging of Adipose Tissue.- Activating Human Adipose Tissue with the ?3-Adrenergic Agonist Mirabegron.- Fluorescent and Luminescent Methods to Detect Lipolysis.- Metabolic Turnover Studies to Quantify Energy Uptake by Thermogenic Adipose Tissues of Mice.- Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes.- Activation of UCP1-Independent Ca2+ Cycling Thermogenesis by Wireless Optogenetics.- Measurement of Futile Creatine Cycling Using Respirometry.- Analysis of Enhancers and Transcriptional Networks in Thermogenic Adipocytes.- Diet-Induced Thermogenesis: Principles and Pitfalls.- Fluorescent Genetic Tools for Studying Brown Fat Development and Function in Mice.- Isolation and Characterization of Human Brown Adipocytes.- Setting Ambient Temperature Conditions to Optimize Translation of Molecular Work from the Mouse to Human: The 'Goldilocks Solution'.- Signaling Lipidomic Analysis of Thermogenic Adipocytes.- Blood Sampling for Arteriovenous Difference Measurements Across Interscapular Brown Adipose Tissue in Rat.- In Vivo Imaging of Brown Adipose Tissue in Humans: FDG-PET/CT and Beyond.- Analysis of Single Cell/Nucleus Transcriptome Data in Adipose Tissue.
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BAT biology;Metabolic fitness;Thermogenic adipocytes;Therapeutic stimulation;Thermogenesis