Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods

Enzyme- or light-triggered cyclopropenes for bioorthogonal ligation
Ting Jiang and Scott T. Laughlin

Analysis of metabolically labeled inositol phosphate messengers by NMR
Robert Puschmann, Robert K. Harmel and Dorothea Fiedler

Photo-releasable derivatives of inositol pyrophosphates
Sebastian Hauke, Tamara Bittner, Henning J. Jessen and Carsten Schultz

IMPACT: Imaging phospholipase D activity with clickable alcohols via transphosphatidylation
Timothy W. Bumpus, Dongjun Liang and Jeremy M. Baskin

Fluorescent bioorthogonal labeling of class B GPCRs in live cells
Srikanth Kumar Gangam and Qing Lin

Near-infrared photoactivatable nitric oxide donors with photoacoustic readout
Effie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo and Jefferson Chan

H2S donors with optical responses
Michael D. Pluth, Yu Zhao and Matthew M. Cerda

Visualization of oxidative stress-induced carbonylation in live mammalian cells
Kamalika Mukherjee, Tak Ian Chio and Susan L. Bane

Receptor-based fluorescent sensors constructed from ribonucleopeptide
Skun Nakano, Hiroaki Konishi and Takashi Morii

Conformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopy
Siddharth S. Matikonda, Ralph Goetz, Ryan McLaughlin, Markus Sauer and Martin J. Schnermann

A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophore
Michael P. Luciano, Saghar Nourian, Alexander P. Gorka, Roger R. Nani, Tadanobu Nagaya, Hisataka Kobayashi and Martin J. Schnermann

Quantitative measurement of cytosolic penetration using the chloroalkane penetration assay
Kirsten Deprey and Joshua A. Kritzer

Cell-penetrating and mitochondrion-targeting molecules
George Appiah Kubi and Dehua Pei

A photocaged DNA nanocapsule for delivery and manipulation in cells
Yihong Feng, Takeshi Tohgasaki, Yasuyuki Shitomi, Hiroshi Sugiyama and Masayuki Endo

A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells
Esther Braselmann and Amy E. Palmer

Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG)
Kayla N. Busby and Neal K. Devaraj

Assaying RNA solvent accessibility in living cells with LASER
Chao Feng and Robert C. Spitale

Split T7 RNA polymerase biosensors to study multiprotein interaction dynamics
Jeffrey A. Dewey and Bryan C. Dickinson

Cross-linking cellular nucleic acids via a target-directing double click reagent
Masayuki Tera and Nathan W. Luedtke

Click chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probes
Sepideh Tavakoli, Yifang Liu, Jacob Potts and Sara H. Rouhanifard

Quantification of protein delivery in live cells using fluorescence correlation spectroscopy


Susan L. Knox, Angela Steinauer, Garrett Alpha-Cobb, Adam Trexler, Elizabeth Rhoades and Alanna Schepartz

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• Bioquímica
• Biologia molecular

Chemical Tools; Imaging; Manipulating; Tracking; Biological Systems; FDAA Probes; Penicillin-binding Proteins