Mutation Breeding in Coffee with Special Reference to Leaf Rust

Introduction coffee breeding and challenges.- Choice of materials for mutation induction in arabica coffee.- Improved in-vitro establishment and germination of Coffea arabica seed.- Induced mutagenesis in coffee (Coffea arabica L.) using chemical agents.- Mutation induction using gamma irradiation and high frequency embryogenic callus from coffee (Coffea arabica).- Chemical mutagenesis of Coffea arabica mature seed using EMS.- Physical mutagenesis of coffee seeds.- In-vitro regeneration of Coffea arabica var. Venecia through somatic embryogenesis.- Protocol on mutation induction in Coffea arabica using in vivo grafting and cuttings.- Protocol on mutation induction in coffee using in vitro tissue cultures.- Screening for resistance to coffee leaf rust.- Protocol to send samples of coffee leaf rust to CIFC.- Coffee leaf rust (Hemileia vastatrix) inoculation and evaluation under laboratory conditions.- Development of a PCR-Based Molecular Detection.- Technique for the Early Diagnosisof Coffee Leaf.- Rust Caused by Hemileia vastatrix.- Protocols for chromosome preparations: molecular cytogenetics and studying genome organization in coffee.

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• Produção de Agronomia e cultura
• Genética (não médica)
• Botânica e ciências das plantas
• Engenharia genética

Open Access;Coffee Leaf Rust;Mutation Breeding;Coffea arabica;PCR-based molecular detection