Embryo Models In Vitro
portes grátis
Embryo Models In Vitro
Methods and Protocols
Turksen, Kursad; Zernicka-Goetz, Magdalena
Springer-Verlag New York Inc.
02/2024
323
Dura
Inglês
9781071636855
15 a 20 dias
Descrição não disponível.
Generation of Human Blastoids from Naive Pluripotent Stem Cells.- Human Pre-Gastrulation Embryo Culture in 3D Condition.- Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System.- Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models.- Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology.- Endometrial Assembloids to Model Human Embryo Implantation In Vitro.- Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (?PASE).- Generation of 3D Trophoblast Organoids from Human Naive Pluripotent Stem Cells.- Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells.- Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells.- Generation of Stem Cell-Based Mouse Embryo-Like Structures.- Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond.- Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts.- Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast.- Single-Cell mRNA-sncRNA Co-Sequencing of Preimplantation Embryos.- Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas.- Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL.- ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis.- Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence.- Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics.- Whole-Mount RNA, Single Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos.
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Embryo culture;Embryogenesis;Pluripotent stem cells;Embryo-like structures;3D cellular models
Generation of Human Blastoids from Naive Pluripotent Stem Cells.- Human Pre-Gastrulation Embryo Culture in 3D Condition.- Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System.- Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models.- Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology.- Endometrial Assembloids to Model Human Embryo Implantation In Vitro.- Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (?PASE).- Generation of 3D Trophoblast Organoids from Human Naive Pluripotent Stem Cells.- Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells.- Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells.- Generation of Stem Cell-Based Mouse Embryo-Like Structures.- Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond.- Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts.- Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast.- Single-Cell mRNA-sncRNA Co-Sequencing of Preimplantation Embryos.- Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas.- Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL.- ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis.- Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence.- Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics.- Whole-Mount RNA, Single Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos.
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