Adipose-Derived Stem Cells
Adipose-Derived Stem Cells
Methods and Protocols
Frazier, Trivia; Bunnell, Bruce; Sanchez, Cecilia; Gimble, Jeffrey
Springer-Verlag New York Inc.
03/2024
393
Dura
Inglês
9781071637616
15 a 20 dias
Descrição não disponível.
Isolation of Human Adipose-Derived Stem Cells.- Isolation of Huma n Adipose-Derived Stem Cells from Lipoaspirates.- Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry.- Soft Tissue Reconstruction.- Adult Stem Cells Freezing Processes and Cryopreservation Protocols.- Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic and Osteogenic Differentiation or Flow Cytometry-Based Analysis.- Isolation of Murine Adipose-Derived Stromal/Stem Cells using an Explant Culture Method.- Canine Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation, Characterization, and Differentiation.- Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation.- Manufacturing of a Huma Adipose-Derived Hydrogel.- Quality Control Assessment of Human Adipose-Derived Hydrogels.- Analysis of Biomechanical Properties of Adipose-Derived Hydrogelsfor Adipose-Derived Stem Cell Based Hydrogel Culture.- Combination of Adipose Stem Cells and Decellularized Adipose Tissue Hydrogel for Osteogenic Applications.- Preparation of Decellularized Amniotic Membrane and Adipose-Derived Stem Cell Seeding.- Robust Generation of ASC Spheroids for Use as 3D Cultures and in Bioprinted Tissue Models.- Generating and Characterizing Adipose Spheroids from Adipose Stem Cells.- Spheroid Formation of Human Adipose-Derived Stem Cells Using a Liquid-Overlay Technique.- Repurposing Decellularized Lung to Generate Vascularized Fat.- In Vitro Culture of White Adipose Tissue.- Bioreactor Culture to Create Adipose Tissue from Human Mesenchymal Stromal Cells.- A Method for Screening a Kinase Inhibitor Drug Library on ASCs.- Analyses and Utilization of Selectively-Tuned Human Adipose-Derived Stem Cell Exosomes.- Functional Assays in 3D ObaCell (R) Fat-On-A-Chip Cultures: Lipolysis and Glucose Uptake Assays.- Monitoring ADSC Metabolism Using the Seahorse Analyzer.- Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain.- Nuclear Transcription Factor Detection.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
Cell Isolation;Extracellular Matrices;Adipose Depots;Multipotent Stromal Cell Isolation;Soft Tissue Reconstruction
Isolation of Human Adipose-Derived Stem Cells.- Isolation of Huma n Adipose-Derived Stem Cells from Lipoaspirates.- Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry.- Soft Tissue Reconstruction.- Adult Stem Cells Freezing Processes and Cryopreservation Protocols.- Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic and Osteogenic Differentiation or Flow Cytometry-Based Analysis.- Isolation of Murine Adipose-Derived Stromal/Stem Cells using an Explant Culture Method.- Canine Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation, Characterization, and Differentiation.- Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation.- Manufacturing of a Huma Adipose-Derived Hydrogel.- Quality Control Assessment of Human Adipose-Derived Hydrogels.- Analysis of Biomechanical Properties of Adipose-Derived Hydrogelsfor Adipose-Derived Stem Cell Based Hydrogel Culture.- Combination of Adipose Stem Cells and Decellularized Adipose Tissue Hydrogel for Osteogenic Applications.- Preparation of Decellularized Amniotic Membrane and Adipose-Derived Stem Cell Seeding.- Robust Generation of ASC Spheroids for Use as 3D Cultures and in Bioprinted Tissue Models.- Generating and Characterizing Adipose Spheroids from Adipose Stem Cells.- Spheroid Formation of Human Adipose-Derived Stem Cells Using a Liquid-Overlay Technique.- Repurposing Decellularized Lung to Generate Vascularized Fat.- In Vitro Culture of White Adipose Tissue.- Bioreactor Culture to Create Adipose Tissue from Human Mesenchymal Stromal Cells.- A Method for Screening a Kinase Inhibitor Drug Library on ASCs.- Analyses and Utilization of Selectively-Tuned Human Adipose-Derived Stem Cell Exosomes.- Functional Assays in 3D ObaCell (R) Fat-On-A-Chip Cultures: Lipolysis and Glucose Uptake Assays.- Monitoring ADSC Metabolism Using the Seahorse Analyzer.- Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain.- Nuclear Transcription Factor Detection.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.