TGF-Beta Signaling

TGF-Beta Signaling

Methods and Protocols

Zi, Zhike; Liu, Xuedong

Springer-Verlag New York Inc.

03/2022

239

Mole

Inglês

9781071622797

15 a 20 dias

Descrição não disponível.
Absolute Quantification of TGF-? Signaling Proteins Using Quantitative Western Blot.- Fast Quantitation of TGF-? Signaling Using Adenoviral Reporter.- Complex Formation among TGF-b Receptors in Live Cell Membranes Measured by Patch-FRAP.- Branched Proximity Hybridization Assay for the Quantification of Nanoscale Protein-Protein Proximity.- Visualizing Dynamic Changes during TGF-?-Induced Epithelial to Mesenchymal Transition.- Establishment of Embryonic Zebrafish Xenograft Assays to Investigate TGF-? Family Signaling in Human Breast Cancer Progression.- Generating Somatic Knockout Cell Lines with CRISPR-Cas9 Technology to Investigate SMAD Signaling.- CRISPR-Based Screening in Three-Dimensional Organoid Cultures to Identify TGF-? Pathway Regulators.- Optogenetic Control of TGF-? Signaling.- Using Microfluidics and Live Cell Reporters to Dissect the Dynamics of TGF-? Signaling in Mouse Embryonic Stem Cells.- Energy Landscape Analysis of the Epithelial-Mesenchymal Transition Network.- Discrete Logic Modeling of Cell Signaling Pathways.- Mining of Single-Cell Signaling Time-Series for Dynamic Phenotypes with Clustering.- Automated Classification of Cellular Phenotypes Using Machine Learning in Cellprofiler and CellProfiler Analyst.- Live Cell Imaging of Spatiotemporal Ca2+ Fluctuation Responses to Anticancer Drugs.
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TGF-? signaling;Transforming growth factor beta;Quantitative experiments;Computational methods;Cell signaling pathways