Cell-Wide Identification of Metabolite-Protein Interactions

Cell-Wide Identification of Metabolite-Protein Interactions

Ewald, Jennifer C.; Luzarowski, Marcin; Skirycz, Aleksandra

Springer-Verlag New York Inc.

10/2022

260

Dura

Inglês

9781071626238

15 a 20 dias

Descrição não disponível.
Protein-Metabolite Interactions Shape Cellular Metabolism and Physiology.- Affinity Purification Protocol Starting with a Small Molecule as Bait.- Cellular Thermal Shift Assay for the Detection of Small Molecule-Target Interactions in Arabidopsis Cells.- Solvent-Induced Protein Precipitation for Drug Target Discovery.- Identification of Plant Protein-Metabolite Interactions by Limited Proteolysis-Coupled Mass Spectrometry (LiP-MS).- Limited Proteolysis-Mass Spectrometry to Identify Metabolite-Protein Interactions.- Proteome Integral Solubility Alteration (PISA) for High-Throughput Ligand Target Deconvolution with Increased Statistical Significance and Reduced Sample Amount.- Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes Using a Single-Step Affinity Purification.- Detecting and Characterizing Interactions of Metabolites with Proteins by Saturation Transfer Difference Nuclear Magnetic Resonance (STD-NMR) Spectroscopy.- PROMIS: Co-Fractionation Mass Spectrometry for Analysis of Protein-Metabolite Interactions.- Metabolomics: Going Deeper, Going Broader, Going Further.- Specifics of Metabolite-Protein Interactions and Their Computational Analysis and Prediction.- Biophysical Approaches for the Characterization of Protein-Metabolite Interactions.- Databases and Tools to Investigate Protein-Metabolite Interactions.
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Cellular processes;Metabolomics;Proteomics;Protein-small molecule complexes;Computational techniques