Cell Cycle Checkpoints
portes grátis
Cell Cycle Checkpoints
Methods and Protocols
Manfredi, James J.
Springer-Verlag New York Inc.
03/2022
242
Mole
Inglês
9781071612194
15 a 20 dias
493
Descrição não disponível.
Determination of CHK1 Cellular Localization by Immunofluorescence Microscopy.- Detection of Post-Translationally Modified p53 by Western Blotting.- Global Analyses to Identify Direct Transcriptional Targets of p53.- Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay.- Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors.- DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids.- A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer.- The DNA Damage Response in Xenopus laevis Cell-Free Extracts.- Mammalian Cell Fusion Assays for the Study of Cell Cycle Progression by Functional Complementation.- Knockdown of Target Genes by siRNA In Vitro.- Assaying Cell Cycle Status Using Flow Cytometry.- Using TUNEL Assay to Quantitate p53-Induced Apoptosis in Mouse Tissues.- Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast.- Calreticulin Exposure in Mitotic Catastrophe.- Quantification of eIF2? Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy.- Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe.
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Flow cytometric methods;Phosphorylation;Protein Degradation;Transcription;Cdk4/6 complexes
Determination of CHK1 Cellular Localization by Immunofluorescence Microscopy.- Detection of Post-Translationally Modified p53 by Western Blotting.- Global Analyses to Identify Direct Transcriptional Targets of p53.- Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay.- Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors.- DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids.- A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer.- The DNA Damage Response in Xenopus laevis Cell-Free Extracts.- Mammalian Cell Fusion Assays for the Study of Cell Cycle Progression by Functional Complementation.- Knockdown of Target Genes by siRNA In Vitro.- Assaying Cell Cycle Status Using Flow Cytometry.- Using TUNEL Assay to Quantitate p53-Induced Apoptosis in Mouse Tissues.- Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast.- Calreticulin Exposure in Mitotic Catastrophe.- Quantification of eIF2? Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy.- Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
