West Nile Virus

Introduction to West Nile Virus.- Quantification of West Nile virus by Plaque-Forming Assay.- Viral titer quantification of West Nile virus by immunostaining plaque assay.- Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection.- Isolation of Murine Bone Marrow Derived Neutrophils for Infection Modeling.- Methods to study West Nile Virus infection, and the virus-induced inflammation in the brain in a murine model.- Detection of West Nile virus envelope protein in brain tissue with an immunohistochemical assay.- Quantitative Analysis of B Cell Subpopulations in Bone Marrow by Flow Cytometry.- Isolation of exosomes or extracellular vesicles from West Nile Virus-infected N2a cells, Primary Cortical Neurons and Brain Tissues.- Protocol to Study West Nile Virus Infection in Brain Slices In Vitro.- Studying Virus-Host Interactions with CRISPR technology.- Protocol of detection of West Nile Virus in Clinical Samples.- Detection and Analysis of West Nile Virus Structural Protein Genes in Animal Or Bird Samples.- Mosquito surveillance for West Nile Virus.- Molecular surveillance of West Nile virus in mosquitoes and sentinel chickens.- Statistical tools for West Nile Virus Disease Analysis.- Analytical Approaches to Uncover Genetic Associations for Rare Outcomes: Lessons from West Nile Neuroinvasive Disease.- Safety procedures to work with West Nile virus in biosafety level 3 facilities.- Development of antibody-based therapeutics against West Nile virus in plants.

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• Virologia (não médica)

RT-qPCR;CRISPR;immunoassaying assay;pathogenesis;epidemiology