Schizosaccharomyces pombe
portes grátis
Schizosaccharomyces pombe
Methods and Protocols
Petreaca, Ruben C.
Springer-Verlag New York Inc.
11/2024
365
Dura
9781071641675
15 a 20 dias
Descrição não disponível.
Dual Luciferase Reporter Assay in Schizosaccharomyces pombe.- High-Throughput Measurement of Single Fission Yeast Cell Volume Using Fluorescence Exclusion.- Measuring Cell Dimensions in Fission Yeast Using Machine Learning.- Ultrastructure Expansion Microscopy (U-ExM) of the Fission Yeast S. pombe.- Live Imaging of Fission Yeast Single Cell Lineages Using a Microfluidic Device.- Analysis of Cell Wall Mechanics in Fission Yeast.- Pil1 Co-Tethering Assay to Detect Protein-Protein Interactions in the Fission Yeast Schizosaccharomyces pombe.- Bi-Molecular Fluorescence Complementation as a Tool to Study Specific Dynamic Interactions Between Proteins in Fission Yeast.- Mapping Active RNA Polymerases in Proliferating and Quiescent Fission Yeast Cells Using Precision Run-On Sequencing.- Fast Fission Yeast Genome Editing by CRISPR/Cas9 Using Gap Repair and Fluoride Selection.- CRISPR-Cas9 Genome Editing in Auxotrophic and Non-Auxotrophic Fission Yeast Strains.- Proliferation Analyses of Conditional Knockdown Strains Using CRISPR Interference in Fission Yeast.- SIMPLE Method for Plasmid Editing.- Investigating Fission Yeast Mutagenesis Using Canavanine Sensitivity Assays.- A Rapidly Inducible DNA Double-Strand Break to Monitor Telomere Formation, DNA Repair, and Checkpoint Activation.- Targeted Forward Genetics: Saturating Mutational Analyses of Specific Target Loci Within the Genome.- Assessing Drug Sensitivity in Fission Yeast Using Half-Maximal Inhibitory Concentration (IC50) Assays.- ChIPmentation for Epigenomic Analysis in Fission Yeast.- Dissecting the Cell-Killing Mechanisms of Hydroxyurea Using Spot Assays.- Measuring Meiotic Recombination Frequency in Schizosaccharomyces pombe Using an Engineered Genetic Interval.- Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe.- Genome-Wide Profiling of Histone Modifications in Fission Yeast Using CUT&Tag.- Identifying Drug Sensitivities in Fission Yeast by Assessing Growth Kinetics.- An "In Schizo" Evaluation System to Screen for Human Kinesin-5 Inhibitors.- Automated Machine Learning Tools to Build Regression Models for Schizosaccharomyces pombe Omics Data.
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Fission yeast;Eukaryotic biology;CRISPR/Cas9;Microscopy analysis;Genome and plasmid editing;DNA damage;Engineered mutations
Dual Luciferase Reporter Assay in Schizosaccharomyces pombe.- High-Throughput Measurement of Single Fission Yeast Cell Volume Using Fluorescence Exclusion.- Measuring Cell Dimensions in Fission Yeast Using Machine Learning.- Ultrastructure Expansion Microscopy (U-ExM) of the Fission Yeast S. pombe.- Live Imaging of Fission Yeast Single Cell Lineages Using a Microfluidic Device.- Analysis of Cell Wall Mechanics in Fission Yeast.- Pil1 Co-Tethering Assay to Detect Protein-Protein Interactions in the Fission Yeast Schizosaccharomyces pombe.- Bi-Molecular Fluorescence Complementation as a Tool to Study Specific Dynamic Interactions Between Proteins in Fission Yeast.- Mapping Active RNA Polymerases in Proliferating and Quiescent Fission Yeast Cells Using Precision Run-On Sequencing.- Fast Fission Yeast Genome Editing by CRISPR/Cas9 Using Gap Repair and Fluoride Selection.- CRISPR-Cas9 Genome Editing in Auxotrophic and Non-Auxotrophic Fission Yeast Strains.- Proliferation Analyses of Conditional Knockdown Strains Using CRISPR Interference in Fission Yeast.- SIMPLE Method for Plasmid Editing.- Investigating Fission Yeast Mutagenesis Using Canavanine Sensitivity Assays.- A Rapidly Inducible DNA Double-Strand Break to Monitor Telomere Formation, DNA Repair, and Checkpoint Activation.- Targeted Forward Genetics: Saturating Mutational Analyses of Specific Target Loci Within the Genome.- Assessing Drug Sensitivity in Fission Yeast Using Half-Maximal Inhibitory Concentration (IC50) Assays.- ChIPmentation for Epigenomic Analysis in Fission Yeast.- Dissecting the Cell-Killing Mechanisms of Hydroxyurea Using Spot Assays.- Measuring Meiotic Recombination Frequency in Schizosaccharomyces pombe Using an Engineered Genetic Interval.- Label-Free Quantitative Phosphoproteomics in the Fission Yeast Schizosaccharomyces pombe.- Genome-Wide Profiling of Histone Modifications in Fission Yeast Using CUT&Tag.- Identifying Drug Sensitivities in Fission Yeast by Assessing Growth Kinetics.- An "In Schizo" Evaluation System to Screen for Human Kinesin-5 Inhibitors.- Automated Machine Learning Tools to Build Regression Models for Schizosaccharomyces pombe Omics Data.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
