Protein Post-Translational Modifications
Protein Post-Translational Modifications
Methods and Protocols
Zhang, Xu; Li, Jianjun; Chen, Rui
Springer-Verlag New York Inc.
03/2026
320
Dura
Inglês
9781071651650
Pré-lançamento - envio 15 a 20 dias após a sua edição
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An Overview of Mass Spectrometry-Based Methods to Analyze Post-Translational Modifications.- Post-Translational Modifications in Protein Therapeutics: Importance and Analytical Tools.- Decoding the SUMO Proteome: A Mass Spectrometry Workflow for Site-Specific Identification.- Computational Pipelines for Protein Ubiquitylation Analysis and Prediction.- Extensive Characterization of N-Glycosylation from Major Histocompatibility Complex with Hydrophilic Interaction Chromatography and Mass Spectrometry.- Endogenous Phosphopeptidomics Analysis by Using Mesoporous IMAC Materials.- O- and N-Phosphoproteomic Analysis Using Online Alkaline-pH Reversed-Phase Nanoelectrospray-Tandem Mass Spectrometry.- Phosphoproteomics Protocol for the Identification of Novel Biomarkers from Extracellular Vesicles of Cancerous Cells.- Mapping Cellular Protein Lysine Methylation Using Targeted-Mass Spectrometry.- Enrichment and Metaproteomic Analysis of Lysine Acetylation in Fecal Microbiome Samples.- Generation of High-Quality Succinyl Spectral Libraries for Improved Proteome-Wide Succinylome Analysis Using Data-Independent Acquisition.- Combining SDS-PAGE with Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics Analysis of Intact Histone Proteoforms.- PEPPI-SP3: A Gel-Based High-Resolution Sample Preparation Workflow for In-Depth Top-Down Analysis of Intact Proteoforms by Mass Spectrometry.- Profiling O-Acetylation in Sialoglycans Using MALDI-TOF and LC-MS/MS with Methylamidation and Permethylation Derivatization.- Purification of Protein-Free and Protein-Linked Poly(ADP-Ribose) from Biochemical Reactions and from Human Cells.- An NMR-Based Approach for Global Arginine Methylation Analysis.- Nanopore-Based Detection of Protein Post-Translational Modifications.- Mass Spectrometry Analysis of Biotherapeutic Monoclonal Antibody Glycosylation.- Glycoengineering of Monoclonal Antibodies with Homogeneous Glycan Isomers with Asymmetric Antennae.- Deamidation Analysis of Biotherapeutic Proteins by Capillary Electrophoresis Mass Spectrometry.
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PTMs;Protein function;Protein-based therapeutics;Biotherapeutic drug development;Proteoform analysis;Proteomics
An Overview of Mass Spectrometry-Based Methods to Analyze Post-Translational Modifications.- Post-Translational Modifications in Protein Therapeutics: Importance and Analytical Tools.- Decoding the SUMO Proteome: A Mass Spectrometry Workflow for Site-Specific Identification.- Computational Pipelines for Protein Ubiquitylation Analysis and Prediction.- Extensive Characterization of N-Glycosylation from Major Histocompatibility Complex with Hydrophilic Interaction Chromatography and Mass Spectrometry.- Endogenous Phosphopeptidomics Analysis by Using Mesoporous IMAC Materials.- O- and N-Phosphoproteomic Analysis Using Online Alkaline-pH Reversed-Phase Nanoelectrospray-Tandem Mass Spectrometry.- Phosphoproteomics Protocol for the Identification of Novel Biomarkers from Extracellular Vesicles of Cancerous Cells.- Mapping Cellular Protein Lysine Methylation Using Targeted-Mass Spectrometry.- Enrichment and Metaproteomic Analysis of Lysine Acetylation in Fecal Microbiome Samples.- Generation of High-Quality Succinyl Spectral Libraries for Improved Proteome-Wide Succinylome Analysis Using Data-Independent Acquisition.- Combining SDS-PAGE with Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics Analysis of Intact Histone Proteoforms.- PEPPI-SP3: A Gel-Based High-Resolution Sample Preparation Workflow for In-Depth Top-Down Analysis of Intact Proteoforms by Mass Spectrometry.- Profiling O-Acetylation in Sialoglycans Using MALDI-TOF and LC-MS/MS with Methylamidation and Permethylation Derivatization.- Purification of Protein-Free and Protein-Linked Poly(ADP-Ribose) from Biochemical Reactions and from Human Cells.- An NMR-Based Approach for Global Arginine Methylation Analysis.- Nanopore-Based Detection of Protein Post-Translational Modifications.- Mass Spectrometry Analysis of Biotherapeutic Monoclonal Antibody Glycosylation.- Glycoengineering of Monoclonal Antibodies with Homogeneous Glycan Isomers with Asymmetric Antennae.- Deamidation Analysis of Biotherapeutic Proteins by Capillary Electrophoresis Mass Spectrometry.
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