Phytophthora

Phytophthora

Methods and Protocols

Bradshaw, Rosie E.; Gerth, Monica L.

Springer-Verlag New York Inc.

02/2025

263

Dura

9781071643297

Pré-lançamento - envio 15 a 20 dias após a sua edição

Descrição não disponível.
Promoter Analysis and Dissection Using Reporter Genes, Comparative Genomics,



and Gel Shift Assays in Phytophthora.- RNA Silencing Strategies in Phytophthora: Experimental Guidelines and Insights.- Protoplast Transformation of Phytophthora spp.- Gene Editing and Protein Tagging in the Oomycete Phytophthora infestans Using CRISPR-Cas12a.- CRISPR/Cas9-Mediated Gene-Knockout and In Situ Complementation System for Phytophthora sojae.- Fluorescence In Situ Hybridization Protocol for Visualization of Oomycetes In Vitro and In Planta.- Rapid and Robust Monitoring of Phytophthora Infectivity through Detached Leaf Assays with Automated Image Analysis.- Quantification of Phytophthora palmivora Infection in Barley and Related Monocot Roots.- The Use of eDNA Metabarcoding to Detect and Identify Phytophthora in Water Samples.- A Method for the Separation of Phytophthora Oospores from Soil for DNA-Based Detection.- Annotation of RxLR Effectors in Oomycete Genomes.- Pinus radiata cDNA Library for the Screening of Phytophthora Effector Protein Targets in Yeast.- Immunoisolation of Plant Endosomal Vesicles to Explore Uptake of Pathogen Effector Proteins during Infection of Nicotiana benthamiana.- Fabrication of Lab-on-a-Chip Devices to Study the Forces Imparted by Growing Phytophthora Hyphae.- Application of Proteomic Methods in Oomycete Biology.- Heterologous Expression of Secreted Proteins from Phytophthora in Pichia pastoris Followed by Protein Purification by Immobilized Metal Ion Affinity (IMAC).
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
Plant pathogens;Pathogenicity and resistance;Disease quantification;Crop disease;CRISPR-Cas genome editing