From Proteins to Proteomics

Module 1: Basics of proteins and proteomics

Basics of amino acids and proteins

Protein chemistry to proteomics

Module 2: Gel-based proteomics techniques

Gel-based proteomics

2-d difference in gel electrophoresis (2d-dige)

Gel-based proteomic data analysis

Module 3: Basics of mass-spectrometry and quantitative proteomics

Introduction to mass spectrometry

Hybrid mass spectrometry configurations

Tandem mass spectrometry for protein identification

In vitro quantitative proteomics using itraq

In vivo quantitative proteomics using silac

Module 4: Interactomics: basics and application

Introduction to interactomics

Antigen and antibody microarrays

Cell-free expression based protein microarrays

Nucleic acid programmable protein arrays

Label-free proteomics

Surface plasmon resonance

Surface plasmon resonance imaging

Protein interaction analysis using spr and spri

Module 5: Advancement in proteomics

Proteomics for translational research

Future of proteomics for clinical applications

Challenges in clinical proteomics

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• Biologia Celular (citologia)
• Biotecnologia
• Genética (não médica)
• Proteinas
• Biologia molecular

polyacrylamide gel;SDS-PAGE;Mass Spectrometry;Differential Gel Electrophoresis;Gel-based proteomics;SILAC;Protein Microarrays;Clinical Proteomics;DIGE;Nappa;SPRi;Biomolecular Interactions;Mass Analyzer;IPG Strip;Protein Protein Interactions;Mass Spectrometry Based Quantitative Proteomics;Array Surface;Sample Preparation;MS Data Analysis;Cell Free Expression Systems;Studying Protein Protein Interaction;Label Free Technique;SPR;Proteomics Research;Low Abundance Analytes;anti-GST Antibodies;Stable Isotope Labeling;HUPO;Protein Arrays;DIA