Antibiotics

Antibiotics

Methods and Protocols

Sass, Peter

Springer-Verlag New York Inc.

11/2022

405

Dura

Inglês

9781071628546

15 a 20 dias

983

Descrição não disponível.
Antibiotics - precious goods in changing times.- A Whole-Cell Assay for Detection of Antibacterial Activity in Actinomycetes Culture Supernatants.- Sampling of Human Microbiomes to Screen for Antibiotic-Producing Commensals.- Production of Antimicrobial Compounds by Homologous and Heterologous Expression.- Isolation and Purification of Natural Products from Microbial Cultures.- Structure Elucidation of Antibiotics.- Computer-Aided Drug Design - An update.- Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents.- Microscopy-based Multiwell Assay to Characterize Disturbed Bacterial Morphogenesis Upon Antibiotic Action.- Expansion Microscopy of Bacillus subtilis.- Tracking global and local changes in membrane fluidity through fluorescence spectroscopy and microscopy.- Quantitative analysis of microscopy data to evaluate bacterial responses to antibiotic treatment.- Application of a Bacillussubtilis whole-cell biosensor (PliaI-lux) for the identification of cell wall active antibacterial compounds.- Determination of Bacterial Membrane Impairment by Antimicrobial Agents.- A colorimetric assay to identify and characterize bacterial primase inhibitors.- Cell-based fluorescent screen amenable to HTS to Identify Inhibitors of Bacterial Translation Initiation.- -Bacterial two component systems: Overexpression, purification ? in vitro and in vivo inhibitor screens.- Sample preparation for mass-spectrometry based absolute quantification of bacterial proteins in antibiotic stress research.- Elemental analysis for the characterization of antimicrobial effects.- label-free quantitation of ribosomal proteins from bacillus subtilis for antibiotic research.- Functional Metagenomics to Study Antibiotic Resistance.
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Antimicrobial killing mechanisms;Microbial adaptation;Mode of action;Drug susceptibility;Antibiotic targets