Advanced Mammalian Cell Culture Techniques
portes grátis
Advanced Mammalian Cell Culture Techniques
Principles and Practices
Senthilkumar, R.; Hoque, Md Enamul; Ramkumar, K.M.
Taylor & Francis Ltd
10/2023
106
Dura
Inglês
9781032494524
15 a 20 dias
Descrição não disponível.
SECTION 1. BASICS IN CELL CULTURE, 1. Highlights: History of Cell Culture and Its Applications, 2. Aseptic Technique and Essentials in Cell Culture, 3. Laboratory Safety, 4. Preparation of Media for Cell Culture, 5. Primary Cell Culture, 6. Passaging of Cell Lines, 7. Cell or Tissue Culture Models and Cell Line Immortalization, 8. Counting of Cells by Hemocytometer, 9. Cryopreservation of Cells, 10. Thawing of Cells, 11. Detection of Bacterial and Fungal Contamination, 12. Testing for Mycoplasma Contamination, 13. Cellular Cytotoxicity, SECTION 2. ISOLATION AND ANALYSIS OF NUCLEIC ACIDS AND PROTEINS, 14. Isolation of Total RNA and Assessment of Purity and Concentration, 15. Target Gene Amplification and Expression by qRT-PCR, 16. Cell Lysis, Protein Extraction Using RIPA Buffer, 17. Estimation of Protein by Bradford's Assay, 18. SDS-PAGE Analysis, 19. Immunoblotting, SECTION 3. CELL STANING TECHNIQUES, 20. Cell Nuclear Staining by DAPI, 21. Immunofluorescence Staining, 22. Estimation of Intracellular ROS using H2DCFDA Molecular Probes, 23. Analysis of Cell Cycle Regulation and Apoptosis Using Annexin-V and Propidium Iodide (PI) Staining, 24. Estimation of Chemokines and Cytokines Using Bio-Plex Assay, SECTION 4. CELL TRANSFECTION AND SINGLE-CELL ANALYSIS, 25. Reporter Gene Construction and Assay, 26. Vector Transfection and Validation, 27. Single-Cell Analysis
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molecular cloning;immunoblot analysis;qRT-PCR techniques;cell viability assays;laboratory biosafety;protein quantification;advanced single cell methodologies
SECTION 1. BASICS IN CELL CULTURE, 1. Highlights: History of Cell Culture and Its Applications, 2. Aseptic Technique and Essentials in Cell Culture, 3. Laboratory Safety, 4. Preparation of Media for Cell Culture, 5. Primary Cell Culture, 6. Passaging of Cell Lines, 7. Cell or Tissue Culture Models and Cell Line Immortalization, 8. Counting of Cells by Hemocytometer, 9. Cryopreservation of Cells, 10. Thawing of Cells, 11. Detection of Bacterial and Fungal Contamination, 12. Testing for Mycoplasma Contamination, 13. Cellular Cytotoxicity, SECTION 2. ISOLATION AND ANALYSIS OF NUCLEIC ACIDS AND PROTEINS, 14. Isolation of Total RNA and Assessment of Purity and Concentration, 15. Target Gene Amplification and Expression by qRT-PCR, 16. Cell Lysis, Protein Extraction Using RIPA Buffer, 17. Estimation of Protein by Bradford's Assay, 18. SDS-PAGE Analysis, 19. Immunoblotting, SECTION 3. CELL STANING TECHNIQUES, 20. Cell Nuclear Staining by DAPI, 21. Immunofluorescence Staining, 22. Estimation of Intracellular ROS using H2DCFDA Molecular Probes, 23. Analysis of Cell Cycle Regulation and Apoptosis Using Annexin-V and Propidium Iodide (PI) Staining, 24. Estimation of Chemokines and Cytokines Using Bio-Plex Assay, SECTION 4. CELL TRANSFECTION AND SINGLE-CELL ANALYSIS, 25. Reporter Gene Construction and Assay, 26. Vector Transfection and Validation, 27. Single-Cell Analysis
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
