VEGF Signaling

VEGF Signaling

Methods and Protocols

Pellet-Many, Caroline; Fiedler, Lorna R.

Springer-Verlag New York Inc.

04/2022

353

Dura

Inglês

9781071622162

15 a 20 dias

889

Descrição não disponível.
Opposing Roles of Vascular Endothelial Growth Factor C in Metastatic Dissemination and Resistance to Radio/Chemotherapy: Discussion of Mechanisms and Therapeutic Strategies.- Identification of VEGF Isoforms in Mouse, Rat, and Zebrafish Using RT-qPCR.- Multi-Parameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells.- Absolute Quantification of Plasma Membrane Receptors via Quantitative Flow Cytometry.- Measurement of VEGF Content in Exosomes and Subsequent Tumor Tubulogenesis and In Vivo Angiogenesis Functional Assays.- SH2-Domain Protein Isolation Using Synthetic Phosphorylated Peptides to Study VEGFR2 Signaling.- Monitoring VEGF-Stimulated Calcium Ion Flux in Endothelial Cells.- Co-Immunoprecipitation Assays.- Using Immortalized Endothelial Cells to Study the Roles of Adhesion Molecules in VEGF-Induced Signaling.- RNAscope for VEGF-A Detection in Human Tumor Bioptic Specimens.- Global MicroRNA Profiling of Vascular Endothelial Cells.- Endothelial Cell Tube Formation Assay: An In Vitro Model for Angiogenesis.- Use of a Thin Layer Assay for Assessing the Angiogenic Potential of Endothelial Cells In Vitro.- VEGF-A165 -Induced Endothelial Cells Chemotactic Migration and Invasion Assays.- Measuring Mitochondrial Calcium Fluxes in Cardiomyocyte upon Mechanical Stretch-Induced Hypertrophy.- Simultaneous Measurement of Endothelial Cell Proliferation and Cell Cycle Stage Using Flow Cytometry.- Ex Vivo Mouse Aortic Ring Angiogenesis Assay.- Retinal Microvasculature-on-a-Chip for Modeling VEGF-Induced Permeability.- Preventing VEGF-Mediated Vascular Permeability by Experimentally Potentiating BBB Characteristics in Endothelial Cells.- The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesis.- Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay.- Modulation of VEGFA Signaling during Heart Regeneration in Zebrafish.- Three-Dimensional Visualization of Blood and Lymphatic Vessels in the Adult Zebrafish Heart by Chemical Clearing.- Fluorescence-Activated Cell Sorting and Quantitative Real-Time PCR to Reveal VEGF Expressing Macrophage Populations in the Zebrafish Larvae.- Assessing Molecular Regulation of Vascular Permeability Using a VEGF-Inducible Zebrafish Model.
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Vascular Endothelial Growth Factor;VEGF and VEGFR isoforms;Vascular biology;Signaling pathways;Cardiovascular development;Dysregulation