Unfolded Protein Response

Unfolded Protein Response

Methods and Protocols

Perez-Torrado, Roberto

Springer-Verlag New York Inc.

01/2022

332

Dura

Inglês

9781071617311

15 a 20 dias

845

Descrição não disponível.
Measuring cysteine exposure in unfolded proteins with tetraphenylethene maleimide and its analogues.- Fluorescence-based biosensors for the detection of the unfolded protein response.- Indirect methods to measure unfolded proteins in living cells using fluorescent proteins.- A platform technology for monitoring the Unfolded Protein Response.- The use of fluorescent protein fusions to monitor the unfolded protein response and protein foldase-substrate interactions in plant protoplasts.- High-throughput analysis of protein turnover with tandem fluorescent protein timers.- Detection of HAC1 mRNA Splicing by RT-PCR in Saccharomyces cerevisiae.- Determination of protein translation during the activation of the UPR.- Determination of the stability and intracellular (intra-nuclear) targeting and recruitment of pre-HAC1 mRNA in the Saccharomyces cerevisiae during the activation of UPR.- Assays to study IRE1 activation and signaling.- Analysis of XBP1 contribution to hyperosmolarity-induced lipid synthesis.- Integrating an ER stress reporter for monitoring genome wide UPR-ER in budding yeast.- Analyze mouse knockout models of UPR pathway elements.- In vitro stimulation of IRE1a/XBP1-deficient B cells with LPS.- Detection of PERK signaling in the central nervous system.- Methods to study the mitochondrial unfolded protein response (UPRmt) in Caenorhabditis elegans.- Drosophila Unfolded Protein Response (UPR) assays in vitro and in vivo.- Protein preparation for proteomic analysis of the unfolded protein response in Arabidopsis thaliana.- Structure-based drug discovery of IRE1 modulators.- Chemical screening to detect ER stress regulators.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
Tetraphenylethene maleimide;Ire1p activation;mRNA splicing;Transgenic Mouse Models;stress regulators