Rice Genome Engineering and Gene Editing

Rice Genome Engineering and Gene Editing

Methods and Protocols

Thilmony, Roger; Bandyopadhyay, Anindya

Springer-Verlag New York Inc.

01/2022

378

Mole

Inglês

9781071610701

15 a 20 dias

751

Descrição não disponível.
Gene Assembly in Agrobacterium via Nucleic Acid Transfer Using Recombinase Technology (GAANTRY).- TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology.- Morphogenic Regulators and Their Application in Improving Plant Transformation.- A Rapid Method for Stably Transforming Rice Seeds.- Agrobacterium tumefaciens-Mediated Transformation of Rice by Hygromycin Phosphotransferase (hptII) Gene Containing CRISPR/Cas9 Vector.- Genotype Independent Regeneration and Transformation Protocol for Rice Cultivars.- Rapid Vector Construction and Assessment of BE3 and Target-AID C to T Base Editing Systems in Rice Protoplasts.- Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop Improvement.- Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice.- Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole Genome Sequencing.- Efficient Genome Editing in Rice Protoplasts Using CRISPR/CAS9 Construct.- Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice.- Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9.- Rice Haploid Inducer Development by Genome Editing.- FLP-Mediated Site-Specific Gene Integration in Rice.- Rice Gene Targeting by Homologous Recombination with Positive Negative Selection Strategy.- Prediction of Rice Transcription Start Sites Using TransPrise: A Novel Machine Learning Approach.- Single Cell Type Specific RNA Isolation and Gene Expression Analysis in Rice Using Laser Capture Microdissection (LCM)-Based Method.- Immunolocalization Analysis of C4 Proteins in the Leaf Tissue of Rice.- Selection of Suitable Reference Genes for qRT-PCR Gene Expression Studies in Rice.- Rice Protoplast Isolation and Transfection for Transient Gene Expression Analysis.- Identification and Analysis of Steroidogenic Acute Regulatory (StAR) Protein-Related Lipid Transfer (START) Domains in Rice.- Assessing Rice Salinity Tolerance: From Phenomics to Association Mapping.
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CRISPR gene editing;Crop plants;Targeted integration;Gene stacking;Rice production;Transgenic traits;Bioinformatics