RNA Methodologies
-10%
portes grátis
RNA Methodologies
A Laboratory Guide for Isolation and Characterization
Farrell Jr., Robert E.
Elsevier Science & Technology
11/2022
960
Mole
Inglês
9780323902212
15 a 20 dias
Descrição não disponível.
1. RNA and the cellular biochemistry revisited
2. Creating a ribonuclease-free environment 000
3. Stringency: conditions that influence nucleic acid structure
4. Ribonucleic acid isolation strategies
5. Isolation of polyadenylated RNA
6. The truth about tissues
7. Going green: RNA and the molecular biology of plants
8. Quality control for RNA preparations
9. cDNA: a permanent biochemical record of the cell
10. RT-PCR: a science and an art form
11. Quantitative polymerase chain reaction techniques
12. miRNA and other noncoding RNAs
13. RNA interference and gene editing
14. Electrophoresis of RNA
15. Photodocumentation and image analysis
16. Northern analysis
17. Nucleic acid probe technology
18. Quantification of specific mRNAs by nuclease protection
19. Analysis of nuclear RNA
20. RNA in situ hybridization
21. Array analysis of gene expression
22. Subtractive and nonsubtractive methods for the analysis of gene expression
23. Transcriptomes and bioinformatics
24. RNA-seq: the premier transcriptomics tool
25. RNA biomarker discovery and validation
26. Functional genomics strategies
27. A few RNA success stories
Epilogue
Appendix A: Maintaining complete and accurate records
Appendix B: Converting mass to moles
Appendix C: Removal of DNA from an RNA sample
Appendix D: Removal of RNA from a DNA sample
Appendix E: Electrophoresis: principles, parameters, and safety
Appendix F: Disposal of ethidium bromide and SYBR Green solutions
Appendix G: Deionization of formamide, formaldehyde, and glyoxal
Appendix H: Silanizing centrifuge tubes and glassware
Appendix I: Centrifugation as a mainstream tool for the molecular biologist
Appendix J: Dot blot analysis
Appendix K: Useful stock solutions for the molecular biologist
Appendix L: Genomes and proteomes
Appendix M: Common SI prefixes
Appendix N: Common abbreviations
Appendix O: Select suppliers of equipment, reagents, and services
Appendix P: Trademark citations
Glossary
2. Creating a ribonuclease-free environment 000
3. Stringency: conditions that influence nucleic acid structure
4. Ribonucleic acid isolation strategies
5. Isolation of polyadenylated RNA
6. The truth about tissues
7. Going green: RNA and the molecular biology of plants
8. Quality control for RNA preparations
9. cDNA: a permanent biochemical record of the cell
10. RT-PCR: a science and an art form
11. Quantitative polymerase chain reaction techniques
12. miRNA and other noncoding RNAs
13. RNA interference and gene editing
14. Electrophoresis of RNA
15. Photodocumentation and image analysis
16. Northern analysis
17. Nucleic acid probe technology
18. Quantification of specific mRNAs by nuclease protection
19. Analysis of nuclear RNA
20. RNA in situ hybridization
21. Array analysis of gene expression
22. Subtractive and nonsubtractive methods for the analysis of gene expression
23. Transcriptomes and bioinformatics
24. RNA-seq: the premier transcriptomics tool
25. RNA biomarker discovery and validation
26. Functional genomics strategies
27. A few RNA success stories
Epilogue
Appendix A: Maintaining complete and accurate records
Appendix B: Converting mass to moles
Appendix C: Removal of DNA from an RNA sample
Appendix D: Removal of RNA from a DNA sample
Appendix E: Electrophoresis: principles, parameters, and safety
Appendix F: Disposal of ethidium bromide and SYBR Green solutions
Appendix G: Deionization of formamide, formaldehyde, and glyoxal
Appendix H: Silanizing centrifuge tubes and glassware
Appendix I: Centrifugation as a mainstream tool for the molecular biologist
Appendix J: Dot blot analysis
Appendix K: Useful stock solutions for the molecular biologist
Appendix L: Genomes and proteomes
Appendix M: Common SI prefixes
Appendix N: Common abbreviations
Appendix O: Select suppliers of equipment, reagents, and services
Appendix P: Trademark citations
Glossary
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
?5-bromouridine; AMV reverse transcriptase; Absorbance ratio; Accession number; Aerial tissue; Affinity chromatography; Agarose; Aggressive disruption; Anchor PCR; Annealing; Antisense; Aptamer; Assay sensitivity; Autoradiography; BLAST search; Bark RNA; Base-pairing; Beadbeater; Biohazard; Bioinformatics; Biomarker; Biomarker signature; Biomolecular imager; Biopharma; Biotin; Biotin-labeled probe; Branched DNA (bDNA); CDNA; CEL-seq; COVID-19; CP-RNA-seq; CRISPR; CRISR-Cas9; CTAB; Canonical base-pairing; Capillary transfer; CaptureSeq; Cell wall; ChIP; ChIP-seq; Chaotropic lysis; Chemiluminescence; Chloroplast RNA; Chromatin immunoprecipitation (ChIP); CircRNA; Circulating nucleic acids; Clinical manifestation; Computational bioinformatics; Contig; Coronavirus; Cotyledon RNA; CsCl; CsTFA; Cy3; Cy5; Cytoplasmic RNA; DNA; Denaturing; Detection; Digital image analysis; Digoxigen-labeled probe; Digoxigenin; Disease detection; Double-stranded; Dounce; Downregulation; Driver cDNA; DropSeq; Duplex; EST; Early detection; Electroblot; Electrophoresis; Electrophoretic profile; Electrophoretogram; Enhancement; Enrichment; Epigenetics; Ethidium bromide (EtBr); Exosome; Exosome isolation; Expression profile; FISH; Filtration; First-strand cDNA; Formaldehyde; Formamide; Fruit RNA; Functional genomics; GRO-seq; Gel; Gene expression; Gene knockdown; Gene mapping; Genome; Genome-wide association studies (GWAS); Genomic; Global analysis; Graphical array; Guide RNA
1. RNA and the cellular biochemistry revisited
2. Creating a ribonuclease-free environment 000
3. Stringency: conditions that influence nucleic acid structure
4. Ribonucleic acid isolation strategies
5. Isolation of polyadenylated RNA
6. The truth about tissues
7. Going green: RNA and the molecular biology of plants
8. Quality control for RNA preparations
9. cDNA: a permanent biochemical record of the cell
10. RT-PCR: a science and an art form
11. Quantitative polymerase chain reaction techniques
12. miRNA and other noncoding RNAs
13. RNA interference and gene editing
14. Electrophoresis of RNA
15. Photodocumentation and image analysis
16. Northern analysis
17. Nucleic acid probe technology
18. Quantification of specific mRNAs by nuclease protection
19. Analysis of nuclear RNA
20. RNA in situ hybridization
21. Array analysis of gene expression
22. Subtractive and nonsubtractive methods for the analysis of gene expression
23. Transcriptomes and bioinformatics
24. RNA-seq: the premier transcriptomics tool
25. RNA biomarker discovery and validation
26. Functional genomics strategies
27. A few RNA success stories
Epilogue
Appendix A: Maintaining complete and accurate records
Appendix B: Converting mass to moles
Appendix C: Removal of DNA from an RNA sample
Appendix D: Removal of RNA from a DNA sample
Appendix E: Electrophoresis: principles, parameters, and safety
Appendix F: Disposal of ethidium bromide and SYBR Green solutions
Appendix G: Deionization of formamide, formaldehyde, and glyoxal
Appendix H: Silanizing centrifuge tubes and glassware
Appendix I: Centrifugation as a mainstream tool for the molecular biologist
Appendix J: Dot blot analysis
Appendix K: Useful stock solutions for the molecular biologist
Appendix L: Genomes and proteomes
Appendix M: Common SI prefixes
Appendix N: Common abbreviations
Appendix O: Select suppliers of equipment, reagents, and services
Appendix P: Trademark citations
Glossary
2. Creating a ribonuclease-free environment 000
3. Stringency: conditions that influence nucleic acid structure
4. Ribonucleic acid isolation strategies
5. Isolation of polyadenylated RNA
6. The truth about tissues
7. Going green: RNA and the molecular biology of plants
8. Quality control for RNA preparations
9. cDNA: a permanent biochemical record of the cell
10. RT-PCR: a science and an art form
11. Quantitative polymerase chain reaction techniques
12. miRNA and other noncoding RNAs
13. RNA interference and gene editing
14. Electrophoresis of RNA
15. Photodocumentation and image analysis
16. Northern analysis
17. Nucleic acid probe technology
18. Quantification of specific mRNAs by nuclease protection
19. Analysis of nuclear RNA
20. RNA in situ hybridization
21. Array analysis of gene expression
22. Subtractive and nonsubtractive methods for the analysis of gene expression
23. Transcriptomes and bioinformatics
24. RNA-seq: the premier transcriptomics tool
25. RNA biomarker discovery and validation
26. Functional genomics strategies
27. A few RNA success stories
Epilogue
Appendix A: Maintaining complete and accurate records
Appendix B: Converting mass to moles
Appendix C: Removal of DNA from an RNA sample
Appendix D: Removal of RNA from a DNA sample
Appendix E: Electrophoresis: principles, parameters, and safety
Appendix F: Disposal of ethidium bromide and SYBR Green solutions
Appendix G: Deionization of formamide, formaldehyde, and glyoxal
Appendix H: Silanizing centrifuge tubes and glassware
Appendix I: Centrifugation as a mainstream tool for the molecular biologist
Appendix J: Dot blot analysis
Appendix K: Useful stock solutions for the molecular biologist
Appendix L: Genomes and proteomes
Appendix M: Common SI prefixes
Appendix N: Common abbreviations
Appendix O: Select suppliers of equipment, reagents, and services
Appendix P: Trademark citations
Glossary
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
?5-bromouridine; AMV reverse transcriptase; Absorbance ratio; Accession number; Aerial tissue; Affinity chromatography; Agarose; Aggressive disruption; Anchor PCR; Annealing; Antisense; Aptamer; Assay sensitivity; Autoradiography; BLAST search; Bark RNA; Base-pairing; Beadbeater; Biohazard; Bioinformatics; Biomarker; Biomarker signature; Biomolecular imager; Biopharma; Biotin; Biotin-labeled probe; Branched DNA (bDNA); CDNA; CEL-seq; COVID-19; CP-RNA-seq; CRISPR; CRISR-Cas9; CTAB; Canonical base-pairing; Capillary transfer; CaptureSeq; Cell wall; ChIP; ChIP-seq; Chaotropic lysis; Chemiluminescence; Chloroplast RNA; Chromatin immunoprecipitation (ChIP); CircRNA; Circulating nucleic acids; Clinical manifestation; Computational bioinformatics; Contig; Coronavirus; Cotyledon RNA; CsCl; CsTFA; Cy3; Cy5; Cytoplasmic RNA; DNA; Denaturing; Detection; Digital image analysis; Digoxigen-labeled probe; Digoxigenin; Disease detection; Double-stranded; Dounce; Downregulation; Driver cDNA; DropSeq; Duplex; EST; Early detection; Electroblot; Electrophoresis; Electrophoretic profile; Electrophoretogram; Enhancement; Enrichment; Epigenetics; Ethidium bromide (EtBr); Exosome; Exosome isolation; Expression profile; FISH; Filtration; First-strand cDNA; Formaldehyde; Formamide; Fruit RNA; Functional genomics; GRO-seq; Gel; Gene expression; Gene knockdown; Gene mapping; Genome; Genome-wide association studies (GWAS); Genomic; Global analysis; Graphical array; Guide RNA
