Protein Downstream Processing
portes grátis
Protein Downstream Processing
Design, Development, and Application of High and Low-Resolution Methods
Labrou, Nikolaos E.
Springer-Verlag New York Inc.
11/2021
511
Mole
Inglês
9781071607770
15 a 20 dias
994
Descrição não disponível.
Protein Purification Technologies.- High Throughput Process Development: I. Process Chromatography.- 3. High Throughput Process Development: II. Membrane Chromatography.- Media Selection in Ion Exchange Chromatography in a Single Microplate.- High Throughput Screening of Dye-Ligands for Chromatography.- Technical Optimizations for the High Throughput Purification of Antibodies on Automated Liquid Handlers.- Aqueous Two-Phase Systems for Clean-Up and Recovery of Enzymes from Plants and Plant-Derived Extracts.- Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins.- Recombinant Proteins Co-Expressed and Co-Purified in the Presence of Antibody Fragments.- Affinity Tags in Protein Purification and Peptide Enrichment: An Overview.- Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins.- Zbasic - A Purification Tag for Selective Ion-Exchange Recovery.- An Orthogonal Fusion Tag for Efficient Protein Purification.- Biomimetic Affinity Ligands for Protein Purification.- Synthesis and Evaluation of Dye-Ligand Affinity Adsorbents for Protein Purification.- Design of Affinity Chromatography Peptide Ligands through Combinatorial Peptide Libraries Screening.- ZCa: A Protein A-Derived Domain with Calcium-Dependent Affinity for Mild Antibody Purification.- Macroporous Polymer Monoliths for Affinity Chromatography and Solid Phase Enzyme Processing.- Sample Displacement Batch Chromatography of Proteins.- Lectin Affinity Chromatography: An Efficient Method to Purify Horse IgG3.- Heparin-Binding Affinity Tag: A Novel Affinity Tag for Simple and Efficient Purification of Recombinant Proteins.- Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+.- Antibody Aggregate Removal using a Mixed-Mode Chromatography Resin.- Proteomic Analysis of Food Allergens by MALDI TOF/TOF Mass Spectrometry.- Protein Structure Analysis and Validation with X-Ray Crystallography.- Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes.- Stabilization of Proteins by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin.- G Protein-Coupled Receptor Expression and Purification.- (Hyper)thermophilic Enzymes: Production and Purification.- Screening of Recombinant Lignocellulolytic Enzymes through Rapid Plate Assays.
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Process Chromatography;Membrane Chromatography;ion exchange;dye-ligands;microscale chromatography pipette tips;bioinformatics;Two-Phase Systems
Protein Purification Technologies.- High Throughput Process Development: I. Process Chromatography.- 3. High Throughput Process Development: II. Membrane Chromatography.- Media Selection in Ion Exchange Chromatography in a Single Microplate.- High Throughput Screening of Dye-Ligands for Chromatography.- Technical Optimizations for the High Throughput Purification of Antibodies on Automated Liquid Handlers.- Aqueous Two-Phase Systems for Clean-Up and Recovery of Enzymes from Plants and Plant-Derived Extracts.- Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins.- Recombinant Proteins Co-Expressed and Co-Purified in the Presence of Antibody Fragments.- Affinity Tags in Protein Purification and Peptide Enrichment: An Overview.- Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins.- Zbasic - A Purification Tag for Selective Ion-Exchange Recovery.- An Orthogonal Fusion Tag for Efficient Protein Purification.- Biomimetic Affinity Ligands for Protein Purification.- Synthesis and Evaluation of Dye-Ligand Affinity Adsorbents for Protein Purification.- Design of Affinity Chromatography Peptide Ligands through Combinatorial Peptide Libraries Screening.- ZCa: A Protein A-Derived Domain with Calcium-Dependent Affinity for Mild Antibody Purification.- Macroporous Polymer Monoliths for Affinity Chromatography and Solid Phase Enzyme Processing.- Sample Displacement Batch Chromatography of Proteins.- Lectin Affinity Chromatography: An Efficient Method to Purify Horse IgG3.- Heparin-Binding Affinity Tag: A Novel Affinity Tag for Simple and Efficient Purification of Recombinant Proteins.- Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+.- Antibody Aggregate Removal using a Mixed-Mode Chromatography Resin.- Proteomic Analysis of Food Allergens by MALDI TOF/TOF Mass Spectrometry.- Protein Structure Analysis and Validation with X-Ray Crystallography.- Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes.- Stabilization of Proteins by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin.- G Protein-Coupled Receptor Expression and Purification.- (Hyper)thermophilic Enzymes: Production and Purification.- Screening of Recombinant Lignocellulolytic Enzymes through Rapid Plate Assays.
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