Methods in Stem Cell Biology - Part A

1. Orthotopic brain tumor models derived from glioblastoma stem-like cells
Katharina M. Eyme, Alessandro Sammarco, and Christian E. Badr
2. Identification and isolation of slow-cycling glioma stem cells
Liam Furst, Ryan J. Atkins, Marija Dinevska, Stanley S. Stylli, Niall M. Corcoran, Christopher M. Hovens, and Theo Mantamadiotis
3. Immunohistochemical analysis of the developing mouse cortex
Mohamad-Reza Aghanoori, Kaylan M.L. Burns, Maneesha Subha, Laura Williams, Michelle Hua, Farzaneh Nobakht, Taylor Krawec, and Guang Yang
4. Subcellular fractionation of brain tumor stem cells
Ahmad Sharanek, Laura Raco, Vahab D. Soleimani, and Arezu Jahani-Asl
5. microRNAs transcriptional profiling of mammary stem cells isolated by PKH26 staining and FACS sorting
Chiara Tordonato, Matteo Jacopo Marzi, Pier Paolo Di Fiore, and Francesco Nicassio
6 Genome-wide RNA interference screen in cancer stem cells
Guillaume Pinna, Marie Vandamme, Celia Rouault, Emmanuelle Charafe-Jauffret, and Christophe Ginestier
7. Clonal analysis of human clonogenic keratinocytes
Elena Enzo, Camilla Cattaneo, Federica Consiglio, Maria Pia Polito, Sergio Bondanza, and Michele De Luca
8. Immunofluorescence analysis of myogenic differentiation
Atsushi Asakura and Nobuaki Kikyo
9. Generating pancreatic beta-like cells from human pluripotent stem cells
Lillian Yuxian Lim, Carmen Ching, Dewei Kong, Shiao-Yng Chan, and Adrian Kee Keong Teo
10. In vitro preparation of human Dental Pulp Stem Cell grafts with biodegradable polymer scaffolds for nerve tissue engineering
Jose Ramon Pineda, Yurena Polo, Beatriz Pardo-Rodriguez, Jon Luzuriaga, Veronica Uribe-Etxebarria, Patricia Garcia-Gallastegui, Jose Ramon Sarasua, Aitor Larranaga and Gaskon Ibarretxe
11. Identification of progenitor cells and their progenies in adult Drosophila midgut
Zhen Jin, Minhua Che, and Rongwen Xi
12. Generation and characterization of human mesenchymal stem/stromal cells for cell therapy applications
Luciana M. Dominguez, Esteban J. Fiore, and Guillermo D. Mazzolini
13. Generation of sex-reversed female clonal mice via CRISPR/Cas9-mediated Y chromosome deletion in male embryonic stem cells
Yiren Qin, Fuqiang Geng, and Duancheng Wen

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• Biologia molecular
• Biologia do desenvolvimento
• Biologia Celular (citologia)

ALDH; Beta cell; Biodegradable polymers; Bone marrow; Brain tumor stem cells; Breast cancer; Cancer stem cells; Cell and gene-therapy; Cell lineage tracing; Cell migration; Cerebral cortex; Clonal analysis; CRISPR/Cas9; Culture MSCs; Dental Pulp Stem Cells; Development; Diabetes; Differentiation; Drosophila; Enteroblast (EB); Enterocyte; Enteroendocrine cell (EE); Enteroendocrine progenitor cell (EEP); Epidermal stem cells; FACS-sorting; GAL4/UAS system; Genome-wide RNA interference library; Glioblastoma; Glioma stem cells; GSC; High-content imaging; High-throughput screening; Holoclone; hPSC; HT analysis; Immunofluorescence; Immunohistochemistry; Insulin secretion; Intestinal stem cell (ISC); Intravital dye; iPS; Islet; Kidney capsule transplant; Mammalian brain; Mammospheres; Meroclone; Mesenchymal stem/stromal cells; miRNAs; Mitochondria; Monosomic XO female mice; Mouse embryonic stem cells; MSCs isolation; Myoblast; Myogenesis; Myotube; Nanopatterning; Neural regeneration; Neural stem/precursor cells; Neurodifferentiation; Normal and cancer mammary stem cells; Oregon Green; Pancreas; Paraclone; Patient-derived xenograft mouse models; PKH26 dye; Quiescent; Satellite cells; Skeletal muscle; Slow-cycling; Stem cell; Stem cell therapy; Stem cells; Subcellular fractionation; Tetraploid complementation; Therapeutic resistance; Tissue engineering; TLDA platform; Tumor heterogeneity; Umbilical cord; Y chromosome deletion