Fluorescence In-Situ Hybridization (FISH) for Microbial Cells

Fluorescence In-Situ Hybridization (FISH) for Microbial Cells

Methods and Concepts

Almeida, Carina; Azevedo, Nuno F.

Springer-Verlag New York Inc.

02/2021

334

Dura

Inglês

9781071611142

15 a 20 dias

841

Descrição não disponível.
An Introduction to Fluorescence in situ Hybridization in Microorganisms.- FISH Variants.- Bioinformatic Tools and Guidelines for the Design of Fluorescence in situ Hybridization Probes.- FISH in Suspension or in Adherent Cells.- Application of Nucleic Acid Mimics in Fluorescence in situ Hybridization.- Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes.- Characterisation of Social Interactions and Spatial Arrangement of Individual Bacteria in Multi-Strain or Multispecies Biofilm Systems using Nucleic acid mimics-Fluorescence in situ Hybridization.- Leaf-FISH: In Situ Hybridization Method for Visualizing Bacterial Taxa on Plant Surfaces.- CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) for Complex Environmental Samples.- Fluorescence in Situ Hybridization with Quantum Dot Labels in E. coli Cells.- Monitoring Bacteriophage Infection on Bacterial Cells using FISH.- Linking Microbes to their Genes at Single Cell Level with direct-geneFISH.- Assigning Function To Phylogeny: FISH-nanoSIMS.- Assigning Function to Phylogeny: MAR-FISH.- Counting mRNA Copies in Intact Bacterial Cells by Fluctuation Localization Imaging-based Fluorescence in situ hybridization (fliFISH).- Integration of FISH and Microfluidics.- Flow-FISH using Nucleic Acid Mimic Probes for the Detection of Bacteria.- FISH in Food Samples.- Extraction of Microbial Cells from Environmental Samples for FISH Approaches.- Quality Control in Diagnostic Fluorescence In Situ Hybridization (FISH) in Microbiology.- Computational Resources and Strategies to Construct Single-Molecule Models of FISH.
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FISH;permeabilization;PNA;NanoSIMS;FRET-ISH