Essential Enzyme Kinetics

1. Introduction 2. Basic Chemical Kinetics 3. Initial-Rate Kinetics of One-Substrate Enzymes 4. Measuring Initial Velocities of Enzyme-catalyzed Reactions 5. Multi-substrate Enzyme Kinetic Mechanisms 6. Fast Kinetic Techniques for Probing Enzyme-Catalyzed Reactions 7. Factors Affecting Enzyme Rates 8. Kinetic Isotope Effects 9. Inhibitor Effects on Enzyme-Catalyzed Reactions 10. Enzyme Cooperativity 11. Kinetics of Force-Generating Enzymes

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• Bioquímica
• Biofísica
• Biologia molecular
• Farmacologia

Acid-base catalysis; Activator effects; Actoclampin molecular motors; Adair-Koshland cooperativity model; Adenylosuccinate synthetase; Alanine-scanning mutagenesis; Alcohol dehydrogenase; Arrhenius theory; ATP synthase; Barriers to reaction; Bisubstrate rate experiments; Briggs-Haldane treatment; Brownian motors; Carbonic anhydrase; Catalytic rate enhancement; Chemical relaxation theory; Concept of reaction mechanisms; Concerted transition cooperativity model; Conformational flexibility; Consecutive first-order reactions; Covalent catalysis; Covalent intermediates; Creatine kinase; Dihydrofolate reductase; DNA processivity clamp loaders; EcoRI endonuclease; Electrostatics; Equilibrium isotope effects; First-order reactions; Flash photolysis; Fluorescence spectroscopy; Force-generating multisubstrate enzymes; Galactokinase; Glutamine synthetase; Heavy isotope effects; Hemoglobin"s O2 binding cooperativity; Hexokinase; Hill cooperativity model; Hysteretic enzymes; Initial-rate methods; Inosine 5'-monophosphate dehydrogenase; Integrated rate laws; Intrinsic binding energy; Isomechanisms; Isotope exchange; Isotopic tracers; Isozymes; K- and V-systems; Kinetics versus dynamics; Ligand cooperativity; Lineweaver-Burk plot; Manometry; Masked kinetic isotope effects; Mass spectroscopy; Meaning of kcat and Vm; Meaning of kcat/Km; Meaning of KS and Km; Metal ion roles in enzymes; Metal ions as enzyme activators; Michaelis-Menten treatment; Molecular motor movements; Monod-Wyman-Changeux cooperativity model; Motor processivity; MWC binding equations; Nonspecific ligand binding; Nuclear magnetic resonance; Nucleoside 5'-diphosphate kinase; Opposing first-order reactions; Ordered ternary complex mechanism; Orienting reactive groups; pH effects; pH measurement; Ping-Pong Bi-Bi mechanism; Polarimetry and circular dichroism; Power stroke; Pressure effects; Pressure-jump techniques; Primary kinetic isotope effects; Proximity effects; Pseudo-second order reactions; Quantum mechanical tunneling; Radiometric assays; Rapid mix/quench technique; Rapid-equilibrium random mechanism; Reactant desolvation; Reactant ground state destabilization; Reaction coordinate diagrams; Reaction energetics; Reaction rate equations; Relaxation methods; Reversible activators; Secondary kinetic isotope effects; Second-order reactions; Sequential cooperativity model; Site-directed mutagenesis; Smoluchowski equation; Specific ligand binding; Steady-state assumption; Stopped-flow technique; Substrate transfer tunnels; Sulfotransferases; Temperature effects; Temperature-jump technique; Theorell-Chance mechanism; Timescales of chemical processes; Transition-state theory; Triosephosphate isomerase; Triosephosphate isomerase catalysis; UV/visible absorption spectroscopy; Work equivalent of ATP"s PO bond; Zero-point energy