Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology

Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology

Wurtzel, Eleanore T

Elsevier Science & Technology

07/2022

574

Dura

Inglês

9780323913539

15 a 20 dias

450

Descrição não disponível.
1. Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches
2. Enzymatic isomerization of ?-carotene mediated by the heme-containing isomerase Z-ISO
3. Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes
4. Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids
5. Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages
6. Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis
7. The isolation of sub-chromoplast structures from tomato and capsicum fruit
8. Carrot protoplasts as a suitable method for protein subcellular localization
9. High throughput production and characterization of carotenoid enzymes for structural and functional studies
10. Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation
11. Characterizing CYTOCHROME P450 Enzymes Involved in Plant Apocarotenoid Metabolism by Using Engineered Yeast System
12. Preparation of carotenoid cleavage dioxygenases for crystallography
13. Heme-dependent enzymes involved in carotenoids biosynthesis in plants
14. Tracking sub-plastidic localization of carotenoid metabolic enzymes with proteomics
15. Golden Rice and lessons learned for inspiring future metabolic engineering strategies and synthetic biology solutions
16. Genetic transformation of Cassava to improve carotenoids: a protocol revisited
17. Elevating fruit carotenoid content in apple (Malus x domestica Borkh)
18. Engineering microbial synthesis of carotenoids
19. The breeder's tool-box for enhancing the content of esterified carotenoids in wheat: from extraction and profiling of carotenoids to marker-assisted selection of candidate genes
20. Drawing on enzyme promiscuity to create novel carotenoids via non-phytoene precursors
21. Plant abscisic acid receptor agonists and antagonists
22. A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi
23. Designing biosensors from a plant apocarotenoid receptor
24. Imaging Retinaldehyde-Protein Binding in Plants using a Merocyanine Reporter 25. Use of directed enzyme evolution to create novel biosynthetic pathways for production of non-natural and rare carotenoids
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15-Cis-?-carotene isomerase (Z-ISO); Abscisic acid; Age-related macular degeneration; Agonist; Agrobacterium transformation; Agrobacterium tumefaciens; Antagonist; Apocarotenoid; Apocarotenoid, click chemistry, drought; Apocarotenoids; Apple; Astaxanthin; Beta-carotene; BiFC; Biosensor for carotenoid precursor; BN-PAGE; C. glutamicum; Carotenogenesis; Carotenoid; Carotenoid-modifying enzymes; Carotenoids; Carotenoids biosynthesis; Carrot cell suspension; Cassava Agrobacterium-mediated transformation; Cassava tissue culture; Chemical biology; Chloroplast; Chloroplasts; Chromoplasts; Cleavage dioxygenase; Cobalt; Confocal microscopy; CRISPR/Cas; CRISPRi library; Crop improvement; Crystallization; Crystallography; Cytochrome P450; Daucus carota; Directed evolution; E. coli; Embryogenic callus; Endosperm specific promoter; Enzyme; Enzymes; Enzymology; EPR; Esterification; Ferredoxin; Fluorescence; Fluorescence polarization; Fluorogenic probes; FNR; Fractionation; Fruit; Gene editing; Genetic engineering; Genetic transformation of clonal crops; Genetically-modified organism; Genome-wide association study; Golden Rice; Heme; Heterologous complementation; Heterologous expression; High-performance liquid chromatography; High-throughput biology; Hordeum chilense; Iisomerase; In planta transient gene expression; Integral membrane proteins; Iron; Isomerase; Isoprenoids; Localization; Lutein; Malus x domestica; Mass spectrometry; Metabolic engineering; Metabolomics; Metalloenzyme; Micronutrient deficiency in staple root crops; Natural variation; Nicotiana benthamiana; Non-heme iron; Non-natural; Pathway engineering; Photosynthesis engineering; Plant development; Plastoglobule; Plastoglobuli; Pome; Post-translational regulation; Protein purification; Protein-protein interaction; Proteomics; Protoplasts; Provitamin A; Pull-down; QTL mapping; Redox-dependent ligand switching; Remobilization; Retinal-binding proteins; Retinoids; Rice (Oryza sativa L.); Spectroscopy; Structural biology; Subcellular localization; Synthetic biology; WAT11; Wheat; Xanthophyll acyl transferase; X-ray crystallography; Y2H; Yeast; Yellow-flesh; Zea mays; ss-Carotene