Bacterial Persistence

Bacterial Persistence

Methods and Protocols

Verstraeten, Natalie; Michiels, Jan

Springer-Verlag New York Inc.

10/2022

292

Mole

Inglês

9781071616239

15 a 20 dias

Descrição não disponível.
Studying Bacterial Persistence: Established Methods And Current Advance.- Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases.- A Robust Method For Generating, Quantifying, and Testing Large Numbers of Escherichia coli persisters.- Enrichment of Persister Cells through ?-lactam-induced Filamentation and Size Separation.- Methods for Enrichment of Bacterial Persister Populations For Phenotypic Screens And Genomic Studies.- Observing Bacterial Persistence at Single-Cell Resolution.- Phenotypic Characterization of Antibiotic Persisters at the Single-Cell Level: From Data Acquisition to Data Analysis.- Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation.- Counting Chromosomes in Individual Bacteria to Quantify their Impacts on Persistence.- Analyzing PERSISTER PROTEOMES with SILAC and Label-Free Methods.- Defining Proteomic Signatures To Predict Multi-Drug Persistence in Pseudomonas aeruginosa.- The use of Experimental Evolution to Study The Response of Pseudomonas aeruginosa to Single or Double Antibiotic Treatment.- Detecting persister awakening determinants.- Monitoring Persister Resuscitation with Flow Cytometry.- Stimulating Aminoglycoside Uptake to Kill Staphylococcus aureus persisters.- In vitro Models for the Study of the Intracellular Activity Of Antibiotics.- Analysis of Salmonella Persister Population Sizes, Dynamics Of Gut Luminal Seeding, And Plasmid Transfer In Mouse Models Of Salmonellosis.- Studying Antibiotic Persistence During Infection.















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Microfluidics;flow cytometry;Metabolomics;aminoglycosides;population dynamics