Advanced Optical Methods for Brain Imaging

Introduction.- Optical Coherence Tomography for Brain Imaging.- Light-sheet microscopy for whole brain imaging.- The Airyscan detector from ZEISS: Confocal Microscopy Evolution for the Neurosciences.- Recording Ca++ Transients in Neurons by TCSPC FLIM.- In vivo imaging of all cortical layers and hippocampal CA1 pyramidal cells by two-photon excitation microscopy.- Patterned two-photon illumination for high-speed functional imaging of brain networks in vivo.- Holographic functional calcium imaging of neuronal circuit activity.- Advanced miniature microscopy for Brain Imaging.- Stimulated Raman scattering microscopy for brain imaging: basic principle, measurements and applications.- Super resolving approaches suitable for brain imaging applications.- Super resolution STED and STORM/PALM microscopy for brain imaging.- Expansion microscopy for brain imaging.- Adaptive Optics in Multiphoton Microscopy.- Chemical processing of brain tissues for large-volume, high-resolution optical imaging.

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• Laser física
• Biotecnologia
• Análise de espectro, spectrochemistry, espectrometria de massa
• Engenharia electrónica
• Processamento de sinais
• Biofísica
• Engenharia biomédica

Superresolution microscopy;STED microscopy;STORM microscopy;Light Sheet microscopy;FLIM/FRET microscopy;Multiphoton microscopy;SRS microscopy;Adaptive Optics;Artificial Neural Network;Ultrasensitive fluorescent proteins;Optically Controllable Proteins;Whole-brain functional imaging